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Department of Biochemical and Chemical Engineering

July 2020

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The image shows the methodology for transferring (bio-)chemistry from Batch to Continuous Flow. © AD​/​BCI

July 2020 - Methodology for transferring (bio-)chemistry from Batch to Continuous Flow

The coiled flow inverter (CFI) is a promising reactor design providing enhanced heat and mass transfer, narrow residence time distribution, and rapid mixing for a methodology for the transfer of (bio-)chemical reactions in batch mode to two-phase continuous flow. First, this methodology is used for current development of a droplet-based reaction screening system, which was first tested with an automated Paal-Knorr pyrrole synthesis as model reaction. Second, we investigated the bio-catalyzed oxidation of ABTS by the enzyme laccase in a straight capillary for process development in a CFI. An optical evaluation method for determination of reaction rate was used. Comparing Michaelis-Menten kinetic of the batch reaction and the continuous reaction in a capillary, the results show that the batch reaction can be mapped to the capillary setup.

Corresponding publication: Jens Bobers, Julia Gruehn, Stefan Hoeving, Tobias Pyka, Norbert Kockmann Two-phase Flow in Coiled Flow Inverter – Process Development from Batch to Continuous Flow. acs Org. Process Res. Dev. DOI: https://doi.org/10.1021/acs.oprd.0c00152