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Department of Biochemical and Chemical Engineering

March 2020

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The image shows the Design of an Automated Reagent-Dispensing System for Reaction Screening and Validation with DNA-tagged Substrates. © AD​/​BCI

March 2020 - Design of an Automated Reagent-Dispensing System for Reaction Screening and Validation with DNA-tagged Substrates

In cooperation with the medicinal chemistry, an automated system for dispensing reagents for DNA-encoded chemistry based on an open-source 3D printer model was developed. It was validated by applying a complex three-component Povarov reaction from DNA-tagged aldehydes, electron-rich olefins, and anilines to DNA-tagged hexahydropyrroloquinolines. A surface-active polymer was used to mediate the reaction between DNA-tagged aldehydes, the hydrophobic anilines, and the olefin. We tested the reaction with an aniline and olefin, each dissolved as stock in five organic solvents. Head-to-head comparison of manually performed and automatically performed experiments by the robot showed that the ADoS reproduced the manually pipetted reactions faithfully and reached yields above 90 % with a polymer concentration of 500 µM.

Corresponding publication: Bobers, J.; Klika Škopić, M.; Dinter, R.; Sakthithasan, P.; Neukirch, L.; Gramse, C.: Weberskirch, R.; Brunschweiger, A.; Kockmann, N. Design of an Automated Reagent-Dispensing System for Reaction Screening and Validation with DNA-tagged Substrates. ACS Comb. Sci. DOI: https://doi.org/10.1021/acscombsci.9b00207