March 2020
- Picture of the Month
March 2020 - Design of an Automated Reagent-Dispensing System for Reaction Screening and Validation with DNA-tagged Substrates
In cooperation with the medicinal chemistry, an automated system for dispensing reagents for DNA-encoded chemistry based on an open-source 3D printer model was developed. It was validated by applying a complex three-component Povarov reaction from DNA-tagged aldehydes, electron-rich olefins, and anilines to DNA-tagged hexahydropyrroloquinolines. A surface-active polymer was used to mediate the reaction between DNA-tagged aldehydes, the hydrophobic anilines, and the olefin. We tested the reaction with an aniline and olefin, each dissolved as stock in five organic solvents. Head-to-head comparison of manually performed and automatically performed experiments by the robot showed that the ADoS reproduced the manually pipetted reactions faithfully and reached yields above 90 % with a polymer concentration of 500 µM.
Corresponding publication: Bobers, J.; Klika Škopić, M.; Dinter, R.; Sakthithasan, P.; Neukirch, L.; Gramse, C.: Weberskirch, R.; Brunschweiger, A.; Kockmann, N. Design of an Automated Reagent-Dispensing System for Reaction Screening and Validation with DNA-tagged Substrates. ACS Comb. Sci. DOI: https://doi.org/10.1021/acscombsci.9b00207